B, KALYANA BABU and R K, MATHUR and K, SURESH and G, RAVICHANDRAN and B, SUSANTHI and S, TIWARI (2023) In Vitro CULTURAL STUDIES FOR CALLOGENESIS AND EMBRYOGENESIS OF OIL PALM (Elaeis guineensis Jacq.) USING SEEDLING AND SPEAR LEAF EXPLANTS. Journal of Oil Palm Research.
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Abstract
In oil palm, hybrids are not homogeneous due to their highly heterogeneous nature. However, the generation of homogeneous material is of prime importance, which can be possible only by using somatic embryogenesis using explants like spear leaf and seedling leaf. Evaluation of callogenesis and embryogenesis of oil palm using seedling and spear leaf is reported here. This is the first report on in vitro production of callus and somatic embryos using leaves as explants in India. Seedling leaf experiment revealed that callus induction ranged from 3%-4% among the four media combinations used in the study. Somatic embryo induction was observed in modified Murashige and Skoog (MS) containing picloram (37.26 μmol). The minimum time required for callus induction was 29 days whereas the maximum time required for initiation of callus was 5 months and 4 days. The minimum time required for induction of embryogenic callus was noted to be 7 months and 5 days on two different media, whereas, the maximum time was 11 months and 14 days on modified N6 with dicamba (72.39 μmol) (palm no. 377). The present study opens up the prospect of understanding embryogenesis and manipulating in vitro culture conditions to achieve plantlet regeneration in oil palm using leaf explant.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > SB Plant culture |
| Divisions: | Government Publication > Others |
| Depositing User: | Esam @ Hisham Muhammad |
| Date Deposited: | 20 Jan 2025 00:40 |
| Last Modified: | 20 Jan 2025 00:40 |
| URI: | http://odlsystem2.utm.my/id/eprint/5333 |
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