Microbiology techniques

Cress, Aria (2012) Microbiology techniques. Orange Apple, Delhi, India. ISBN 9788132328797

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Abstract

Digital holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. Instead, the light wave front information originating from the object is digitally recorded as a hologram, from which a computer calculates the object image by using a numerical reconstruction algorithm. The image forming lens in traditional microscopy is thus replaced by a computer algorithm. Other closely related microscopy methods to digital holographic microscopy are interferometric microscopy, optical coherence tomography and diffraction phase microscopy. Common to all methods is the use of a reference wave front to obtain amplitude (intensity) and phase information. The information is recorded on a digital image sensor or by a photo detector from which an image of the object is created (reconstructed) by a computer. In traditional microscopy, which do not use a reference object is lost wave front, only intensity information is recorded and essential information about the Digital holography has mostly been applied to light microscopy. However, digital holography has also been applied to electron microscopy. Holography was invented by Dennis Gabor to improve the electron microscope. Digital electron holography may finally bring home holography to its birth place and fulfill Gabor's vision.

Item Type: Book
Subjects: Q Science > QR Microbiology
Divisions: Electronic Books
Depositing User: Esam @ Hisham Muhammad
Date Deposited: 12 Feb 2023 06:47
Last Modified: 12 Feb 2023 06:47
URI: http://odlsystem2.utm.my/id/eprint/3971

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