Kahn, Cleo (2012) DNA replication. Research World, Delhi, India. ISBN 9788132332718
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Abstract
DNA replication, the basis for biological inheritance, is a fundamental process - that occurs in all living organisms - that copies their DNA. This process is replication, in that each strand of the original double-stranded DNA molecule serves as a template for the reproduction of the complementary strand. Therefore, following DNA replication, two identical DNA molecules have been produced from a single double-stranded DNA molecule. Cellular proofreading and error toe-checking mechanisms ensure near perfect fidelity for DNA replication. In a cell, DNA replication begins at specific locations in the genome, called "origins". Unwinding of DNA at the origin, and synthesis of new strands, forms a replication fork. In addition to DNA polymerase, the enzyme that synthesizes the new DNA by adding nucleotides matched to the template strand, a number of other proteins are associated with the fork and assist in the initiation and continuation of DNA synthesis. DNA replication can also be performed in vitro (outside a cell), DNA polymerases, isolated from cells, and artificial DNA primers are used to initiate DNA synthesis at known sequences in a template molecule. The polymerase chain reaction (PCR), a common laboratory technique, employs such artificial synthesis in a cyclic manner to amplify a specific target DNA fragment from a pool of DNA.
| Item Type: | Book |
|---|---|
| Subjects: | Q Science > QP Physiology |
| Divisions: | Electronic Books |
| Depositing User: | Esam @ Hisham Muhammad |
| Date Deposited: | 02 Jan 2023 02:10 |
| Last Modified: | 02 Jan 2023 02:10 |
| URI: | http://odlsystem2.utm.my/id/eprint/3890 |
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